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Aktivitas Antibakteri Kombinasi Ekstrak Etanol Daun Mangga (Mangifera indica L.) dengan Hidroksiapatit dan Organo-Hidroksiapatit terhadap Staphylococcus aureus
Abstract
Pendahuluan: Peningkatan resistensi terhadap antibiotik merupakan masalah serius dalam pengobatan infeksi, terutama yang disebabkan oleh Staphylococcus aureus. Ekstrak daun mangga (Mangifera indica L.) diketahui mengandung senyawa antimikroba, seperti fenolik dan flavonoid, yang dapat menghambat pertumbuhan bakteri. Selain itu, hidroksiapatit (HAp) juga memiliki aktivitas antibakteri melalui interaksi langsung dengan membran sel bakteri. Organo-hidroksiapatit (Organo-HAp), sebagai bentuk modifikasi dari HAp, diharapkan dapat meningkatkan bioaktivitas dan efisiensinya sebagai agen antibakteri. Tujuan: Penelitian ini mengevaluasi aktivitas antibakteri kombinasi ekstrak etanol daun mangga (EEDM) dengan HAp dan Organo-HAp terhadap bakteri S. Aureus. Metode: Metode yang digunakan adalah eksperimen laboratorium melalui difusi kertas cakram. Berbagai variasi larutan dari EEDM, HAp, Organo-HAp, kombinasi EEDM dengan HAp, dan EEDM dengan Organo-HAp disiapkan untuk diuji terhadap S. Aureus. Hasil: Diameter zona hambat diukur setelah masa inkubasi, dan hasilnya menunjukkan bahwa kombinasi EEDM dengan HAp serta Organo-HAp menghasilkan aktivitas antibakteri sedang, dengan diameter zona hambat masing-masing sebesar 5,76 mm dan 6,36 mm. Kesimpulan: Kombinasi EEDM dengan Organo-HAp menunjukkan efek signifikan terhadap pertumbuhan S. aureus, dengan diameter zona hambat yang mengindikasikan potensinya untuk pengembangan lebih lanjut dalam mengatasi infeksi yang disebabkan oleh bakteri tersebut.
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DOI: https://doi.org/10.33085/jdf.v9i2.6273
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